Coding
TCaiiA
Part:BBa_K2471000
Designed by: Armando Palacios Chaparro Group: iGEM17_Tec-Chihuahua (2017-10-17)
(T7 promoter + RBS) + (aiiA) + (T1 terminator)
This BioBrick™ contains the necessary genetic circuitry to constitutively express the aiiA gene, that when expressed, results in the production of the aiiA enzyme, and thus, the quenching of quorum sensing thanks to the decrease in AHLs.
A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 137
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |